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ARTICLE INFORMATION:
Author:
Loh Kwek Leong 
Title:  How I Incubate Lyretails' Eggs

Summary: Lyretail and other non-annuals' eggs fungus easily. Java moss and a ghost shrimp provide a novel solution, as illustrated here in a wonderful series of close-up photographs.
Contact for editing purposes:
email: Loh "Timebomb" at: timebomb@pacific.net.sg

Date first published:  2004
Publication: Loh's web site www.killies.com

Reprinted from Aquarticles:
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How I Incubate Lyretails' Eggs

by Loh Kwek Leong  of Singapore
Visit Loh's www.killies.com for information on killifish keeping.
Aquarticles

Incubated in water, the eggs of the Lyretails tend to fungus easily. Hatching period is about 10 days and during this time, unfertilised eggs will definitely fungus while good eggs quite often turn bad when attacked by bacteria in the water.

Ghostshrimp1.JPG (20417 bytes)

Adding diluted acriflavine and methylene blue to the water can help prevent fungus but getting the dosages right can be quite tricky. Too heavy a dosage will kill the eggs; too light and it won't be effective. Many thanks to Tyrone Genade for the information on his website at "http://tgenade.freeshell.org" and Wright Huntley's post contained therein, I discovered a good method of incubating non-annual eggs.

Ghostshrimp2.JPG (18298 bytes)

The method is simple and has a very high success rate. It involves using Java Moss and a Ghost Shrimp.

Ghostshrimp3.JPG (29988 bytes)   First, I pick the eggs from the spawning mops using an egg-picker, a tool fashioned from a straw.

Ghostshrimp4.JPG (9105 bytes)   I spray painted the egg-picker black as it's easier to see the eggs when they are contrasted against a dark background.

Ghostshrimp5.JPG (12208 bytes)   I put some Java Moss into a plastic container with water from the breeding tanks. I try not to let the eggs drop to the bottom of the container but place them on the fronds of the moss. The moss helps to keep the water clear and free from bacteria.

Ghostshrimp6.JPG (13313 bytes)   After all the eggs have been placed into the container, I introduce a Ghost Shrimp. These shrimp are scavengers. They also eat algae. They are inexpensive and are sold in some local fish shops. In heavily planted tanks, they breed and multiply rapidly. They are also sometimes known as Malayan shrimp.
The shrimp is different from those long-arm shrimp commonly sold in local fish shops as food for big fishes.

Ghostshrimp7.JPG (21905 bytes)   Once introduced into the container, the Ghost Shrimp goes to work almost immediately. It will pick up the eggs and "clean" them.
It doesn't actually "clean" the eggs though. Being a scavenger, it will however eat up the tiny particles that are attached to the eggs.

Ghostshrimp8.JPG (17498 bytes)   Another picture of the Ghost Shrimp cleaning two eggs at one go. The Java Moss, besides keeping the water clear, also makes the shrimp feel safe.

Ghostshrimp9.JPG (9847 bytes)   Day 2 - 24 hours after picking the eggs, unfertilised eggs turn white. Under normal circumstances such eggs will start to fungus, but with the Ghost Shrimp around, the eggs remain clean. The shrimp eats the fungus. It also cleans good eggs regularly, thereby preventing them from turning bad.

Ghostshrimp10.JPG (7060 bytes)   Day 3 - Due to the movements of the Ghost Shrimp, most of the eggs have dropped to the bottom of the container. The unfertilised eggs are clear from fungus.

Ghostshrimp11.JPG (8634 bytes)   Day 4 - The good eggs are beginning to eye up while the two unfertilised eggs are still free from fungus. They look like tiny ping-pong balls.
The shrimp's droppings are all over the bottom of the container. Obviously it's getting a lot of food, in spite of the small container.

Ghostshrimp12.JPG (8100 bytes)   Day 5 - For some strange reason, the two unfertilised eggs in the container have disappeared. It could be the shrimp ate them; I'm not sure.
The eyes on the good eggs have become darker.

Ghostshrimp13.JPG (8357 bytes)   Day 6 - Eggs are still developing. There's no sign of fungus.

Ghostshrimp14.JPG (8207 bytes)   Day 7 - Eggs still looking good.

Ghostshrimp15.JPG (8238 bytes)   Day 8 - Eggs are close to hatching. Everything's looking good.

Ghostshrimp16.JPG (8143 bytes)   Day 9 - Fry at last!!

Not all eggs will hatch at the same time. If eggs are clearly well-developed but won't hatch, force-hatch them.

The shrimp will not eat the fry as it's incapable of catching live fish. It will not eat the good eggs either so don't worry about it having a "caviar festival". The shrimp is a scavenger, not a predator.